ÉϺ£ºãÔ¶ÉúÎïºÍ»ª¶«Ê¦·¶´óѧ¶à´ÎºÏ×÷´øÀ´µÄºÏ×÷³É¹û£¡

ºãÔ¶²úÆ·ÎÄÏ×:´óÊóÒȵºËØ,ÊÝËØ,ÓÎÀëÖ¬·¾ËáELISAÊÔ¼ÁºÐÒýÓÃÎÄÏ×
¡¾ÎÄÏ×±êÌâ¡¿Hyperlipidemia induces typical atherosclerosis development in Ldlr £ánd Apoe deficient rats
¡¾×÷Õß¡¿Yongliang Zhao, Yiqing Yang, Roumei Xing£¬et.al
¡¾×÷Õßµ¥Î»¡¿»ª¶«Ê¦·¶´óѧ£¨East China Normal University£©
¡¾ÎÄÏ×ÖÐÒýÓòúÆ·¡¿
´óÊóÒȵºËØ(INS)ELISAÊÔ¼ÁºÐ
´óÊóÊÝËØ(LEP)ELISAÊÔ¼ÁºÐ
´óÊóÓÎÀëÖ¬·¾Ëá(FFA)ELISAÊÔ¼ÁºÐ
¡¾¹Ø¼ü´Ê¡¿ Apoe; Ldl receptor; atherosclerosis; rat; gene knockout
¡¾DOI¡¿10.1016/j.atherosclerosis.2018.02.015
¡¾Ó°ÏìÒò×Ó(IF)¡¿4.26
¡¾³ö°æÆÚ¿¯¡¿¡¶Atherosclerosis¡·
¡¾²úÆ·Ô­ÎÄÒýÓá¿
Biochemical analysis
103 Rats were fasted overnight (12-14 h) £ánd blood samples from the retro-orbital plexus were
104 collected. Serum was obtained by centrifugation at 3000 rpm for 15 min at 4 °C, then kept
105 frozen at ?80 °C until analysis. Lipids £ánd lipoproteins including total cholesterol (TC),
106 triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein
107 cholesterol (HDL-C), ApoB £ánd lipoprotein (a) were analyzed using AU680 Automatic
108 Biochemistry Analyzer (Beckman Coulter, USA). FPLC lipoprotein profiles were assessed by
109 size-exclusion chromatography on Superose 6 10/300 GL column £ánd AKTA purifier (GE
110 Healthcare) [16]. We also measured liver £ánd kidney indexes such as aspartate
111 aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA) £ánd creatinine levels.
112 Furthermore, the atherosclerosis index £ánd LDL/HDL ratio were calculated. Atherosclerosis
113 index was calculated as [TC- HDL-C]/HDL-C [17]. Serum insulin, leptin £ánd free fatty acid
114 (FFA) levels were measured using ELISA kits (Hengyuan Biological Technology Co. Ltd.,
115 Shanghai, China

ºãÔ¶²úÆ·ÎÄÏ×:Óã±û¶þÈ©£¨MDA£©ELISAÊÔ¼ÁºÐÒýÓÃÎÄÏ×
¡¾ÎÄÏ×±êÌâ¡¿Environmental concentrations of antibiotics impair zebrafish gut health
¡¾×÷Õß¡¿Li Zhou£¬Samwel Mchele Limbu£¬Meilin Shen£¬et.al
¡¾×÷Õßµ¥Î»¡¿»ª¶«Ê¦·¶´óѧ£¨East China Normal University£©
¡¾ÎÄÏ×ÖÐÒýÓòúÆ·¡¿
Óã±û¶þÈ©£¨MDA£©ELISAÊÔ¼ÁºÐ
¡¾¹Ø¼ü´Ê¡¿Antibiotic£¬Intestinal microbiota£¬Gut health£¬Zebrafish
¡¾DOI¡¿https://doi.org/10.1016/j.envpol.2017.12.073
¡¾Ó°ÏìÒò×Ó(IF)¡¿5.98
¡¾³ö°æÆÚ¿¯¡¿¡¶Environmental Pollution¡·
¡¾²úÆ·Ô­ÎÄÒýÓá¿
 Biochemical assays
In order to minimize bias, each treatment contained 30 fish for biochemical analysis. The whole gut contents of each fish were weighed £ánd homogenized with 9 vol (v/w) of 0.8% physiological saline. Then, the homogenate was centrifuged at 2500×g at 4¡æfor 10 min £ánd the supernatant was collected for biochemical assays according to the manufacturer£§s instructions. Malondialdehyde (MDA) was measured using an enzyme-linked immune sorbent assay (ELISA) kit (Hengyuan, Shanghai). Superoxide dismutase (SOD), peroxidase (POD), reduced glutathione (GSH), acid phosphatase (ACP), £ánd alkaline phosphatase (AKP) were measured using related commercial assay kits (Nanjing Jiancheng Institute,China). Results were recorded on a microplate reader (Epoch, BioTek, USA).

ºãÔ¶²úÆ·ÎÄÏ×:ÓãÒȵºËØ£¨Insulin£©ELISAÊÔ¼ÁºÐÒýÓÃÎÄÏ×£¨ÐÂÍøÕ¾ÒÑ·¢£©
¡¾ÎÄÏ×±êÌâ¡¿Inhibited auhagy impairs systemic nutrient metabolism in Nile tilapia
¡¾×÷Õß¡¿Si-Lan Han£¬Jing Wang£¬Yu-Xue Zhang£¬et.al
¡¾×÷Õßµ¥Î»¡¿»ª¶«Ê¦·¶´óѧ£¨East China Normal University£©
¡¾ÎÄÏ×ÖÐÒýÓòúÆ·¡¿
ÓãÒȵºËØ£¨Insulin£©ELISAÊÔ¼ÁºÐ
¡¾¹Ø¼ü´Ê¡¿Auhagy£¬Metabolism£¬Homeostasis£¬Inflammation£¬Nile tilapia
¡¾DOI¡¿https://doi.org/10.1016/j.cbpa.2019.06.021
¡¾Ó°ÏìÒò×Ó(IF)¡¿4.0
¡¾³ö°æÆÚ¿¯¡¿¡¶Comparative Biochemistry £ánd Physiology, Part A¡·
¡¾²úÆ·Ô­ÎÄÒýÓá¿
At the end of trial, all fish were fasted overnight, six fish of each group were euthanized (MS-222 at 20 mg/L) £ánd sampled to collect tissues to measure the molecular, protein £ánd biochemical indexes.Hepatic triglyceride (TG), glycogen, malondialdehyde (MDA), superoxide dismutase (SOD), £ánd serum TG, free fatty acid (FFA), £ánd glucose were assessed by commercial kits (Jiancheng Biotech Co. China).The serum insulin was detected by ELISA kits (Hengyuan Biotech Co.China). Briefly, the total lipid of the whole fish body, liver £ánd muscle was extracted by using chloroform/methanol (2:1, v/v) as previously described (Bligh £ánd Dyer, 1959). Briefly, the samples were homogenized in the mixed chloroform-methanol 2:1 (vol/vol), £ánd the samples were stored at 4 °C for 24-h extraction. Afterwards, the chloroform phase was carefully moved to a clean glass tube £ánd dried using nitrogen, £ánd the extracted total lipid was weighed £ánd recorded.Whole fish protein £ánd muscle protein were measured by Kjeltec? 8200(FOSS, Sweden).

ºãÔ¶²úÆ·ÎÄÏ×:ÓãάÉúËØK1£¨VK1£©ELISAÊÔ¼ÁºÐÒýÓÃÎÄÏ×
¡¾ÎÄÏ×±êÌâ¡¿Disruption of Abcc6 Transporter in Zebrafish Causes Ocular Calcification £ánd Cardiac Fibrosis
¡¾×÷Õß¡¿Jianjian Sun£¬Peilu She£¬Xu Liu£¬et.al
¡¾×÷Õßµ¥Î»¡¿»ª¶«Ê¦·¶´óѧ£¨East China Normal University£©
¡¾ÎÄÏ×ÖÐÒýÓòúÆ·¡¿
ÓãάÉúËØK1£¨VK1£©ELISAÊÔ¼ÁºÐ
¡¾¹Ø¼ü´Ê¡¿PXE£¬abcc6£¬vitamin K£¬ocular calcification£¬cardiac fibrosis
¡¾DOI¡¿https://doi.org/10.3390/ijms22010278
¡¾Ó°ÏìÒò×Ó(IF)¡¿4.55
¡¾³ö°æÆÚ¿¯¡¿¡¶INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES¡·
¡¾²úÆ·Ô­ÎÄÒýÓá¿
4.12. ELISA Assay
This kit used the double antibody sandwich method to determine the level of vitamin K1 (VK1) in fish (Shanghai Hengyuan Biological Technology Co., Ltd., H-42702, Shanghai,China). The monoclonal antibody vitamin K1 was added into the micropore £ánd combined with horseradish peroxidase (HRP) to form a complex. After polyester, tetramethylbenzidine (TMB) was added to develop color. Under TMB catalysis, it was transformed to blue. There was a positive correlation between the color £ánd the fish vitamin K1 in the fish,measured at 450 nm by enzyme-linked immunosorbent assay. The absorbance (OD value)of fish vitamin K1 in the sample was calculated by the standard curve. To determine the cMGP levels, a human matrix gamma carboxyglutamic acid protein (cMGP) detection kit was selected (Shanghai Hengyuan Biological Technology Co., Ltd., H-11621, Shanghai,China). The purified HRP labeled cMGP antibody was coated on the microporous plate to form a complex. After thorough washing, the substrate TMB was added for color rendering.The experiment was repeated three times.

ÍêÕû°æPDFÎÄÏ×Çë×ÉѯÔÚÏß¿Í·þ»òÕߵ绰ÁªÏµÎÒ˾ҵÎñÔ±£¡
¸ü¶à¹«Ë¾¸£ÀûÇë¹Ø×¢“ºãÔ¶ÉúÎï”΢ÐŹ«Öںţ¡
 
  ÉϺ£ºãÔ¶ÉúÎï¿Æ¼¼ÓÐÏÞ¹«Ë¾Òµ¹©Ó¦¸ßÆ·ÖÊÈËELISAÊÔ¼ÁºÐ¡¢´óÊóELISAÊÔ¼ÁºÐ¡¢Ð¡ÊóELISAÊÔ¼ÁºÐ¡¢ëàÊóELISAÊÔ¼ÁºÐ¡¢ÍÃELISAÊÔ¼ÁºÐ¡¢Å£ELISAÊÔ¼ÁºÐµÈ¸÷ÖÖELISAÊÔ¼ÁºÐ¼°ÉúÎïÊÔ¼Á¡¢ÅàÑø»ù¡¢±ê׼Ʒ¡¢¶ÔÕÕÆ·¡¢ÑªÇåµÈÊÔÑéºÄ²Ä£¡ÉϺ£ºãÔ¶¹«Ë¾ÊÇÒ»¼ÒÒµ´ÓÊ“²úÆ·Ñз¢Éú²ú¡¢Êг¡ÏúÊÛ¡¢¼¼Êõ·þÎñ”ΪһÌåµÄ¸ß¿Æ¼¼ÉúÎï¼¼Êõ¹«Ë¾£¬ÔÚ¿ÆѧÑо¿ºÍÒ½ÁƼì²âÓòÏíÓиߵÄÉùÓþ¡£

Ô­´´×÷ÕߣºÉϺ£ºãÔ¶ÉúÎï¼¼Êõ·¢Õ¹ÓÐÏÞ¹«Ë¾